WEKO3
アイテム
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Application of High-Performance Capillary Electrophoresis to Quantification of Products of the Reverse Transcriptase-Polymerase Chain Reaction
https://ncu.repo.nii.ac.jp/records/70
https://ncu.repo.nii.ac.jp/records/70d9eb353f-69b3-4479-b783-1aeea8c156b0
名前 / ファイル | ライセンス | アクション |
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B43-19970300-55.pdf (649.2 kB)
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Item type | 紀要論文2 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2013-02-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Application of High-Performance Capillary Electrophoresis to Quantification of Products of the Reverse Transcriptase-Polymerase Chain Reaction | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | PCR | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | capillary electrophoresis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | gliostatin | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Moriyama, Akihiko
× Moriyama, Akihiko× Matsukawa, Kohei× Matsubara, Minako× Kato, Taiji |
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抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | To apply the high-performance capillary electrophoresis (HPCE) to the quantification of RT-PCR products, the conditions for the routine measurement were established as follows: the reaction mixture after RT-PCR (1 μl) was diluted 20-fold with deionized water and introduced into the capillary tube by electrokinetic injection at -10 kV for 40 sec. Electrophoresis was performed at -13 kV for 21 min. DNA was quantified spectrophotometrically at 260 nm during electrophoresis. This method was applied to the quantification of gliostatin mRNA in A 431 cells and compared to the amount of corresponding protein. The amount of gliostatin mRNA was well correlated with that of the protein (r = 0.733), when RT-PCR products derived from β-actin were used as internal standards. The data indicate that HPCE is useful to quantitate mRNA levels in cells. | |||||
書誌情報 |
en : Annual review 巻 1, p. 55-58, 発行日 1997-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13429329 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11168811 |