@article{oai:ncu.repo.nii.ac.jp:00000070,
 author = {Moriyama, Akihiko and Matsukawa, Kohei and Matsubara, Minako and Kato, Taiji},
 journal = {Annual review},
 month = {Mar},
 note = {To apply the high-performance capillary electrophoresis (HPCE) to the quantification of RT-PCR products, the conditions for the routine measurement were established as follows: the reaction mixture after RT-PCR (1 μl) was diluted 20-fold with deionized water and introduced into the capillary tube by electrokinetic injection at -10 kV for 40 sec. Electrophoresis was performed at -13 kV for 21 min. DNA was quantified spectrophotometrically at 260 nm during electrophoresis. This method was applied to the quantification of gliostatin mRNA in A 431 cells and compared to the amount of corresponding protein. The amount of gliostatin mRNA was well correlated with that of the protein (r = 0.733), when RT-PCR products derived from β-actin were used as internal standards. The data indicate that HPCE is useful to quantitate mRNA levels in cells.},
 pages = {55--58},
 title = {Application of High-Performance Capillary Electrophoresis to Quantification of Products of the Reverse Transcriptase-Polymerase Chain Reaction},
 volume = {1},
 year = {1997}
}