{"created":"2023-05-15T13:15:41.618021+00:00","id":70,"links":{},"metadata":{"_buckets":{"deposit":"ff199a6f-4b16-4d3c-9ca7-df952ac2da04"},"_deposit":{"created_by":12,"id":"70","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"70"},"status":"published"},"_oai":{"id":"oai:ncu.repo.nii.ac.jp:00000070","sets":[]},"author_link":["1505","1502","1503","1504"],"item_10_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"58","bibliographicPageStart":"55","bibliographicVolumeNumber":"1","bibliographic_titles":[{},{"bibliographic_title":"Annual review","bibliographic_titleLang":"en"}]}]},"item_10_description_22":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_description_8":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"To apply the high-performance capillary electrophoresis (HPCE) to the quantification of RT-PCR products, the conditions for the routine measurement were established as follows: the reaction mixture after RT-PCR (1 μl) was diluted 20-fold with deionized water and introduced into the capillary tube by electrokinetic injection at -10 kV for 40 sec. Electrophoresis was performed at -13 kV for 21 min. DNA was quantified spectrophotometrically at 260 nm during electrophoresis. This method was applied to the quantification of gliostatin mRNA in A 431 cells and compared to the amount of corresponding protein. The amount of gliostatin mRNA was well correlated with that of the protein (r = 0.733), when RT-PCR products derived from β-actin were used as internal standards. The data indicate that HPCE is useful to quantitate mRNA levels in cells.","subitem_description_type":"Other"}]},"item_10_source_id_12":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13429329","subitem_source_identifier_type":"ISSN"}]},"item_10_source_id_14":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11168811","subitem_source_identifier_type":"NCID"}]},"item_10_version_type_23":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Moriyama, Akihiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Matsukawa, Kohei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsubara, Minako","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Taiji","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2013-02-23"}],"displaytype":"detail","filename":"B43-19970300-55.pdf","filesize":[{"value":"649.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"B43-19970300-55.pdf","url":"https://ncu.repo.nii.ac.jp/record/70/files/B43-19970300-55.pdf"},"version_id":"10d306db-8b90-49aa-8c49-db6e25fa095d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"capillary electrophoresis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gliostatin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Application of High-Performance Capillary Electrophoresis to Quantification of Products of the Reverse Transcriptase-Polymerase Chain Reaction","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Application of High-Performance Capillary Electrophoresis to Quantification of Products of the Reverse Transcriptase-Polymerase Chain Reaction","subitem_title_language":"en"}]},"item_type_id":"10","owner":"12","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-02-23"},"publish_date":"2013-02-23","publish_status":"0","recid":"70","relation_version_is_last":true,"title":["Application of High-Performance Capillary Electrophoresis to Quantification of Products of the Reverse Transcriptase-Polymerase Chain Reaction"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-06-26T06:23:02.408387+00:00"}