| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-01-18 |
| タイトル |
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タイトル |
Improved clonality detection in B-cell lymphoma using a semi-nested modification of the BIOMED-2 PCR assay for IGH rearrangement: A paraffin-embedded tissue study |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
BIOMED-2 PCR assay |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
re-amplification |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
semi-nested modification |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
B-cell lymphomas |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
formalin-fixed paraffin-embedded tissues |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Sakamoto, Yuma
Masaki, Ayako
Aoyama, Satsuki
Han, Shusen
Saida, Kosuke
Fujii, Kana
Takino, Hisashi
Murase, Takayuki
Iida, Shinsuke
稲垣, 宏
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| 抄録(英) |
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内容記述タイプ |
Abstract |
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内容記述 |
The BIOMED-2 PCR protocol for targeting the IGH gene is widely employed for detecting clonality in B-cell malignancies. Unfortunately, the detection of clonality with this method is not very sensitive when paraffin sections are used as a DNA source. To increase the sensitivity, we devised a semi-nested modification of a JH consensus primer. The clonality detection rates of three assays were compared: the standard BIOMED-2, BIOMED-2 assay followed by BIOMED-2 re-amplification, and BIOMED-2 assay followed by semi-nested BIOMED-2. We tested more than 100 cases using paraffin-embedded tissues of various B-cell lymphomas, and found that the clonality detection rates with the above three assays were 63.9%, 79.6%, and 88.0%, respectively. While BIOMED-2 re-amplification was significantly more sensitive than the standard BIOMED-2, the semi-nested BIOMED-2 was significantly more sensitive than both the standard BIOMED-2 and BIOMED-2 re-amplification. An increase in sensitivity was observed in all lymphoma subtypes examined. In conclusion, tumor clonality may be detected in nearly 90% of B-cell lymphoma cases with semi-nested BIOMED-2. This ancillary assay may be useful when the standard BIOMED-2 fails to detect clonality in histopathologically suspected B-cell lymphomas. |
| 書誌情報 |
en : Pathology International
巻 67,
号 9,
p. 453-460,
発行日 2017-09
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| 出版者 |
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出版者 |
John Wiley & Sons |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
13205463 |
| 関連情報 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
PMID |
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関連識別子 |
28868745 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1111/pin.12566 |
| 権利 |
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権利情報 |
© 2017 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd, This is the peer reviewed version of the following article: Sakamoto, Y., Masaki, A., Aoyama, S., Han, S., Saida, K., Fujii, K., Takino, H., Murase, T., Iida, S. and Inagaki, H. (2017), Improved clonality detection in B-cell lymphoma using a semi-nested modification of the BIOMED-2 PCR assay for IGH rearrangement: A paraffin-embedded tissue study. Pathol Int, 67: 453-460., which has been published in final form at https://doi.org/10.1111/pin.12566. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| 著者版フラグ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
J11-PI-13205463-67-453-ina (503.4 kB)
