| アイテムタイプ |
紀要論文2 / Departmental Bulletin Paper(1) |
| 公開日 |
2013-02-23 |
| タイトル |
|
|
タイトル |
Application of High-Performance Capillary Electrophoresis to Quantification of Products of the Reverse Transcriptase-Polymerase Chain Reaction |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
PCR |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
capillary electrophoresis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
gliostatin |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者 |
Moriyama, Akihiko
Matsukawa, Kohei
Matsubara, Minako
Kato, Taiji
|
| 抄録(英) |
|
|
内容記述タイプ |
Other |
|
内容記述 |
To apply the high-performance capillary electrophoresis (HPCE) to the quantification of RT-PCR products, the conditions for the routine measurement were established as follows: the reaction mixture after RT-PCR (1 μl) was diluted 20-fold with deionized water and introduced into the capillary tube by electrokinetic injection at -10 kV for 40 sec. Electrophoresis was performed at -13 kV for 21 min. DNA was quantified spectrophotometrically at 260 nm during electrophoresis. This method was applied to the quantification of gliostatin mRNA in A 431 cells and compared to the amount of corresponding protein. The amount of gliostatin mRNA was well correlated with that of the protein (r = 0.733), when RT-PCR products derived from β-actin were used as internal standards. The data indicate that HPCE is useful to quantitate mRNA levels in cells. |
| 書誌情報 |
en : Annual review
巻 1,
p. 55-58,
発行日 1997-03
|
| ISSN |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13429329 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11168811 |
B43-19970300-55.pdf (649.2 kB)
